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ussing chambers physiologic instruments  (Physiologic Instruments)

 
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    Physiologic Instruments ussing chambers physiologic instruments
    Ussing Chambers Physiologic Instruments, supplied by Physiologic Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/instruments+ussing+chambers/pmc11995168-212-9-10?v=Physiologic+Instruments
    Average 90 stars, based on 1 article reviews
    ussing chambers physiologic instruments - by Bioz Stars, 2026-07
    90/100 stars

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    Schematic of patient‐derived intestinal organoid acquisition and expansion followed by two functional assays. Created with BioRender.com. qPCR Validation of lentivirus‐mediated shRNA knockdown of FGL2 and CFTR in intestinal organoids. Significance of sample differences was calculated using the one‐tailed student t ‐test ( n = 2 biological replicates). All values are mean ± SD. EV = empty vector. Representative confocal microscopy images of calcein green labelled FIS organoids of a healthy individual and a CF patient, before and 60 min after stimulation with Fsk (5 µM). Scale bar, 200 μm. Quantification of FIS response in wt‐CFTR organoids (ORG‐01(HC)) shown in (C) as area under the curve at 60 min. Significance of sample differences was calculated using two‐way ANOVA ( n = 2 biological replicates). All values are mean ± SD. Quantification of FIS response in F508del‐CFTR organoids (ORG‐BX‐002 (dF/dF)) shown in (C) as area under the curve at 60 min. Significance of sample differences was calculated using two‐way ANOVA ( n = 2 biological replicates). All values are mean ± SD. Tracing of <t>Ussing</t> chamber measurements of the transepithelial current produced by 2D monolayer cultures of specified organoids. The F508del‐homozygous monolayers with the knockdowns have been rescued with Trikafta.
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    Schematic of patient‐derived intestinal organoid acquisition and expansion followed by two functional assays. Created with BioRender.com. qPCR Validation of lentivirus‐mediated shRNA knockdown of FGL2 and CFTR in intestinal organoids. Significance of sample differences was calculated using the one‐tailed student t ‐test ( n = 2 biological replicates). All values are mean ± SD. EV = empty vector. Representative confocal microscopy images of calcein green labelled FIS organoids of a healthy individual and a CF patient, before and 60 min after stimulation with Fsk (5 µM). Scale bar, 200 μm. Quantification of FIS response in wt‐CFTR organoids (ORG‐01(HC)) shown in (C) as area under the curve at 60 min. Significance of sample differences was calculated using two‐way ANOVA ( n = 2 biological replicates). All values are mean ± SD. Quantification of FIS response in F508del‐CFTR organoids (ORG‐BX‐002 (dF/dF)) shown in (C) as area under the curve at 60 min. Significance of sample differences was calculated using two‐way ANOVA ( n = 2 biological replicates). All values are mean ± SD. Tracing of <t>Ussing</t> chamber measurements of the transepithelial current produced by 2D monolayer cultures of specified organoids. The F508del‐homozygous monolayers with the knockdowns have been rescued with Trikafta.
    Ussing Chamber Physiologic Instruments Model P2300, supplied by Physiologic Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Schematic of patient‐derived intestinal organoid acquisition and expansion followed by two functional assays. Created with BioRender.com. qPCR Validation of lentivirus‐mediated shRNA knockdown of FGL2 and CFTR in intestinal organoids. Significance of sample differences was calculated using the one‐tailed student t ‐test ( n = 2 biological replicates). All values are mean ± SD. EV = empty vector. Representative confocal microscopy images of calcein green labelled FIS organoids of a healthy individual and a CF patient, before and 60 min after stimulation with Fsk (5 µM). Scale bar, 200 μm. Quantification of FIS response in wt‐CFTR organoids (ORG‐01(HC)) shown in (C) as area under the curve at 60 min. Significance of sample differences was calculated using two‐way ANOVA ( n = 2 biological replicates). All values are mean ± SD. Quantification of FIS response in F508del‐CFTR organoids (ORG‐BX‐002 (dF/dF)) shown in (C) as area under the curve at 60 min. Significance of sample differences was calculated using two‐way ANOVA ( n = 2 biological replicates). All values are mean ± SD. Tracing of Ussing chamber measurements of the transepithelial current produced by 2D monolayer cultures of specified organoids. The F508del‐homozygous monolayers with the knockdowns have been rescued with Trikafta.

    Journal: Molecular Systems Biology

    Article Title: CFTR interactome mapping using the mammalian membrane two‐hybrid high‐throughput screening system

    doi: 10.15252/msb.202110629

    Figure Lengend Snippet: Schematic of patient‐derived intestinal organoid acquisition and expansion followed by two functional assays. Created with BioRender.com. qPCR Validation of lentivirus‐mediated shRNA knockdown of FGL2 and CFTR in intestinal organoids. Significance of sample differences was calculated using the one‐tailed student t ‐test ( n = 2 biological replicates). All values are mean ± SD. EV = empty vector. Representative confocal microscopy images of calcein green labelled FIS organoids of a healthy individual and a CF patient, before and 60 min after stimulation with Fsk (5 µM). Scale bar, 200 μm. Quantification of FIS response in wt‐CFTR organoids (ORG‐01(HC)) shown in (C) as area under the curve at 60 min. Significance of sample differences was calculated using two‐way ANOVA ( n = 2 biological replicates). All values are mean ± SD. Quantification of FIS response in F508del‐CFTR organoids (ORG‐BX‐002 (dF/dF)) shown in (C) as area under the curve at 60 min. Significance of sample differences was calculated using two‐way ANOVA ( n = 2 biological replicates). All values are mean ± SD. Tracing of Ussing chamber measurements of the transepithelial current produced by 2D monolayer cultures of specified organoids. The F508del‐homozygous monolayers with the knockdowns have been rescued with Trikafta.

    Article Snippet: Non‐perfused Ussing chamber , Physiologic Instruments , N/A.

    Techniques: Derivative Assay, Functional Assay, Biomarker Discovery, shRNA, Knockdown, One-tailed Test, Plasmid Preparation, Confocal Microscopy, Produced

    Journal: Molecular Systems Biology

    Article Title: CFTR interactome mapping using the mammalian membrane two‐hybrid high‐throughput screening system

    doi: 10.15252/msb.202110629

    Figure Lengend Snippet:

    Article Snippet: Non‐perfused Ussing chamber , Physiologic Instruments , N/A.

    Techniques: Recombinant, Plasmid Preparation, Cloning, Construct, Transduction, Sequencing, Negative Control, Purification, cDNA Synthesis, Transfection, Protease Inhibitor, Western Blot, Membrane, Software